Cloned (Comment) | Organism |
---|---|
gene FEH-I, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 | Cichorium intybus |
gene FEH-IIa, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 | Cichorium intybus |
gene FEH-IIb, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 | Cichorium intybus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of purified recombinant isozyme Ci1-FEH IIa | Cichorium intybus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cichorium intybus | Q93X59 | - |
- |
Cichorium intybus | Q93X60 | - |
- |
Cichorium intybus | Q9FNS9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography and ultrafiltration | Cichorium intybus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-kestose + H2O | - |
Cichorium intybus | beta-D-fructose + sucrose | - |
? | |
1-kestose + H2O | best substrate | Cichorium intybus | beta-D-fructose + sucrose | - |
? | |
fructofuranosylnystose + H2O | - |
Cichorium intybus | ? | - |
? | |
fructofuranosylnystose + H2O | best substrate | Cichorium intybus | ? | - |
? | |
fructofuranosylnystose + H2O | high activity | Cichorium intybus | ? | - |
? | |
inulin + H2O | best substrate | Cichorium intybus | ? | - |
? | |
inulin + H2O | high activity | Cichorium intybus | ? | - |
? | |
additional information | hydrolysis of Jerusalem artichoke tuber by immobilized isozyme Ci1-FEH IIa and analysis of carbohydrate profile. The fructan in the tuber is rapidly degraded to fructose by immobilized recombinant Ci1-FEH IIa. Substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH IIa has much higher activity than all other investigated FEH isozymes from Cichorium intybus and Helianthus tuberosus | Cichorium intybus | ? | - |
? | |
additional information | substrate specificity, overview. No activity with sucrose and levan | Cichorium intybus | ? | - |
? | |
additional information | substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH I shows quite low activity compared to other FEH isozymes analyzed from Cichorium intybus and Helianthus | Cichorium intybus | ? | - |
? | |
nystose + H2O | - |
Cichorium intybus | ? | - |
? | |
nystose + H2O | best substrate | Cichorium intybus | ? | - |
? | |
nystose + H2O | high activity | Cichorium intybus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ci1-FEH I | - |
Cichorium intybus |
Ci1-FEH IIa | - |
Cichorium intybus |
Ci1-FEH IIb | - |
Cichorium intybus |
fructan 1-exohydrolase I | - |
Cichorium intybus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Cichorium intybus |
40 | 50 | - |
Cichorium intybus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | over 60% of maximal activity within this range | Cichorium intybus |
20 | 70 | over 60% of maximal activity within this range | Cichorium intybus |
25 | 45 | over 50% of maximal activity within this range | Cichorium intybus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Cichorium intybus |
6 | - |
- |
Cichorium intybus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | over 80% of maximal activity within this range | Cichorium intybus |
4 | 6.5 | over 50% of maximal activity within this range | Cichorium intybus |
4 | 7 | over 40% of maximal activity within this range | Cichorium intybus |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis of deduced amino acid sequences of plant fructan metabolism genes | Cichorium intybus |